EnglishCancella i cookie per ripristinare le impostazioni di lingua associate al browser in uso
Titolo/Abstract/Parole chiave

Sintesi e Purificazione di Oligonucleotidi e Nucleosidi 2’- funzionalizzati

Mari, Lara (2009) Sintesi e Purificazione di Oligonucleotidi e Nucleosidi 2’- funzionalizzati. Tesi di Dottorato , Università degli studi di Ferrara.

[img]
Anteprima
File PDF
Tesi_dottorato_Dott._Lara_Mari.pdf

Download (7MB) | Anteprima

    Abstract

    Oligonucleotides (ON) are commonly used as research reagents to modulate gene expression in cell culture and in animal models. Various chemistries of ON have been synthesized, incorporating modifications to the phosphodiester backbone, sugar or nucleobases portions. Oligonucleotides 2’-O-Methyl-RNA-Phosphorothioates (2’-OMePS) can modulate gene expression through splicing arrest of pre-mRNA and inducing the skipping of specific exons. In these years, we have set up a fully equipped laboratory and we have developed efficient and reliable protocols for the synthesis and purification of 2’-OMePS full length oligonucleotides. All synthesis were performed on a GE ÄKTATM oligopilot plus 10 fully automated synthesizer in the 1 to 50 μmol scale range using a polystyrene-based solid support at different loading, and β-cyanoethyl phosphoroamidite synthons. All purification were performed on a ÄKTA basic UPC HPLC system by reverse phase chromatography. Furthermore, we have studied the synthesis of news 2’-O-bile acids (AB) conjugated nucleosides, because in future we will insert these building blocks in the automated synthesis process to obtain different 2’-Oconjugates oligonucleotides containing one or more units of AB for improvement of cellular uptake efficiency.

    Tipologia del documento:Tesi di Dottorato (Tesi di Dottorato)
    Data:13 Marzo 2009
    Relatore:Benetti, Simonetta
    Coordinatore ciclo:Gilli, Gastone
    Istituzione:Università degli studi di Ferrara
    Dottorato:XXI Anno 2006 > SCIENZE CHIMICHE
    Struttura:Dipartimento > Chimica
    Soggetti:Area 03 - Scienze chimiche > CHIM/06 Chimica organica
    Parole chiave:sintesi in fase solida, oligonucleotidi, nucleosidi, acidi biliari
    Depositato il:05 Lug 2009 15:25

    Staff:

    Accesso riservatoAccesso riservato