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High Glucose Induces Adipogenic Differentiation of Muscle-Derived Stem Cells

Aguiari, P. (2009) High Glucose Induces Adipogenic Differentiation of Muscle-Derived Stem Cells. PhD Thesis , Università degli Studi di Ferrara.

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    Regeneration of mesenchymal tissues depends on a resident stem cell population, that in most cases remains elusive in terms of cellular identity and differentiation signals. We here show that primary cell cultures derived from adipose tissue or skeletal muscle differentiate into adipocytes when cultured in high glucose. High glucose induces ROS production and PKCβ activation. These two events appear crucial steps in this differentiation process that can be directly induced by oxidizing agents and inhibited by PKCβ siRNA silencing. The differentiated adipocytes, when implanted in vivo, form viable and vascularized adipose tissue. Overall, the data highlight a previously uncharacterized differentiation route triggered by high glucose that drives not only resident stem cells of the adipose tissue but also uncommitted precursors present in muscle cells to form adipose depots. This process may represent a feed‐forward cycle between the regional increase in adiposity and insulin resistance that plays a key role in the pathogenesis of diabetes mellitus.

    Item Type:Thesis (PhD Thesis)
    Date:2 March 2009
    Tutor:Rizzuto, R.
    Coordinator:Borea, P.A.
    Institution:Università degli Studi di Ferrara
    Divisions:Dipartimento > Medicina clinica e sperimentale
    Subjects:Area 06 - Scienze mediche > MED/04 Patologia generale
    Uncontrolled Keywords:adipose tissue, stem cells, hyperglycemia, reactive oxygen species, protein kinase C
    Deposited on:07 Aug 2009 08:16


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