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Titolo/Abstract/Parole chiave

High Glucose Induces Adipogenic Differentiation of Muscle-Derived Stem Cells

Aguiari, P. (2009) High Glucose Induces Adipogenic Differentiation of Muscle-Derived Stem Cells. Tesi di Dottorato , Università degli Studi di Ferrara.

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    Abstract

    Regeneration of mesenchymal tissues depends on a resident stem cell population, that in most cases remains elusive in terms of cellular identity and differentiation signals. We here show that primary cell cultures derived from adipose tissue or skeletal muscle differentiate into adipocytes when cultured in high glucose. High glucose induces ROS production and PKCβ activation. These two events appear crucial steps in this differentiation process that can be directly induced by oxidizing agents and inhibited by PKCβ siRNA silencing. The differentiated adipocytes, when implanted in vivo, form viable and vascularized adipose tissue. Overall, the data highlight a previously uncharacterized differentiation route triggered by high glucose that drives not only resident stem cells of the adipose tissue but also uncommitted precursors present in muscle cells to form adipose depots. This process may represent a feed‐forward cycle between the regional increase in adiposity and insulin resistance that plays a key role in the pathogenesis of diabetes mellitus.

    Tipologia del documento:Tesi di Dottorato (Tesi di Dottorato)
    Data:2 Marzo 2009
    Relatore:Rizzuto, R.
    Coordinatore ciclo:Borea, P.A.
    Istituzione:Università degli Studi di Ferrara
    Dottorato:XXI Anno 2006 > FARMACOLOGIA E ONCOLOGIA MOLECOLARE
    Struttura:Dipartimento > Medicina clinica e sperimentale
    Soggetti:Area 06 - Scienze mediche > MED/04 Patologia generale
    Parole chiave:adipose tissue, stem cells, hyperglycemia, reactive oxygen species, protein kinase C
    Depositato il:07 Ago 2009 08:16

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