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Titolo/Abstract/Parole chiave

NEW INSIGHTS OF MIR-145 FUNCTION AND REGULATION IN HUMAN BREAST CANCER.

Spizzo, R. (2009) NEW INSIGHTS OF MIR-145 FUNCTION AND REGULATION IN HUMAN BREAST CANCER. Tesi di Dottorato , Università degli Studi di Ferrara.

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    Abstract

    miR-145 is down-regulated in the majority of human cancers, including breast cancer (BC). However, its role remains largely unknown. Here, I provide evidence for miR-145 induced anti-proliferative and pro-apoptotic effect in several BC cell lines, which was not detected in BC cells lacking a functional TP53 gene and exhibiting an estrogen receptor alfa (ESR1) negative status. I found that miR-145 anti-proliferative effects were dependent upon TP53 activation and that activation of TP53 could in turn stimulates miR-145 expression. I also found that miR-145 could repress the expression of ESR1 protein by direct interaction with two sites within its gene coding sequence. My findings support the existence of a positive regulatory loop where miR-145 directly targets ESR1 and indirectly activates TP53, which in turn sustains miR-145 expression and reinforces miR-145 overall effects on proliferation and apoptosis.

    Tipologia del documento:Tesi di Dottorato (Tesi di Dottorato)
    Data:2 Marzo 2009
    Relatore:Croce, C.M.
    Coordinatore ciclo:Borea, P.A.
    Istituzione:Università degli Studi di Ferrara
    Dottorato:XXI Anno 2006 > FARMACOLOGIA E ONCOLOGIA MOLECOLARE
    Struttura:Dipartimento > Medicina clinica e sperimentale
    Soggetti:Area 06 - Scienze mediche > MED/06 Oncologia medica
    Parole chiave:miR-145, breast cancer, estrogen receptor 1, TP53
    Depositato il:06 Ago 2009 10:28

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